Dissolved Primer pellet in pure ethanol instead of water - (Mar/09/2011 )
I accidentally grabbed the ethanol bottle instead of the water one. The ethanol bottle has 100% ethanol. I noticed the stock solution didn't freeze at -20 C and I didn't get any results for my PCR reaction. I was wondering if I can just pipette out the ethanol and add the appropriate amount of water into the primer tubes. I can still see the pellet, but some of the pellet has gotten bigger in area. I really appreciate any help in advance! Thank you!
-Moebius-
why don't you let the ethanol evaporate then resuspend the pellet in water. that way you shouldn't lose any.
you can speed up the evaporation with a speedvac or n-evap.
-mdfenko-