FBS recommendation for HEK293T cells - HEK293T maitenance (Mar/07/2011 )

dear all,
i just have ordered HEK293T cells from openbiosystems, Catalog number: HCL4517

in the manual they recommend certain FBS type (Thermo Scientific Hyclone No.: 30014.03), but the minimum amount that can be ordered is 100l and i will never use it off, since we actually just plan to include the cells in our cell line library. My question is: have you experience with this type of cells? could you recommend another lot of FBS? or do you know how to get a trying exemplar of the recommended one?

thank a lot for any help

-lora-

HEK293T are not very sensitive to the kind/batch of FBS that you use. We even use NCS, that works fine as well, the cells still grow very well. Just use your usual serum should do it.

-Rsm-

thanks a lot that will simplify my life a bit:-)

-lora-

It's also not unusual to test serum batches before you buy them. That is, you can ask them to provide you a "sample" of that particular serum. So you can try it (compare to your normal serum). And then you suddenly realize that it's not good at all, and don't have buy the 100l.

-Rsm-

yeah i had this idea too :rolleyes: and ordered one yesterday

-lora-

sounds like a convincing alternative

lora on Wed Mar 9 08:06:47 2011 said:

yeah i had this idea too :rolleyes:

and ordered one yesterday

-damon-