Do you know good sequencing companies? - Sanger sequencing (Mar/04/2011 )
Hi there...
Thanks everybody for their replies.
Just a short update: I looked at their website and it seems like that Macrogen has sequencing facilities in the US as well. So if you are in the US, then that would mean no shipping to Korea.
Cheers,
V
-Wolverena-
pito on Mon Mar 7 21:52:07 2011 said:
BioMiha on Mon Mar 7 20:36:02 2011 said:
pito on Mon Mar 7 12:51:27 2011 said:
pDNA on Sun Mar 6 11:27:02 2011 said:
I would prefere a company that is somehow near your lab (if possible on the same continent) ...so you do not have to send samples all around the world (allthough some people do send samples for sanger sequencing e.g. from Europe to Korea and that is a bit weird to me). Normally, sanger sequencing is a state of the art technique and read lengths >1000bp are more or less standard ...i think it is most important to purify the DNA in a good way because when it is of poor quality the sequencing will fail no matter what company you have choosen. Regards,p
BioMiha on Sat Mar 5 11:47:24 2011 said:
Macrogen
Dude, in my opinion you're just overdoing it a bit. I am all for eco, I just wanted to say there are better places to start, especially if saving a buck can help run a few additional sequences, get more results and eventually contribute in a small way. I would say going to work on foot or by bike instead of in your car or the subway saves more CO2 emissions than sending your samples to Germany instead of Korea. Not that I am in any way affiliated to the company, but they have cheaper prices because they have state-of-the-art robot sequencers not because they have cheap labor. We are talking about Korea, not China.
-BioMiha-
Don't get offended ...DUDE!
BioMiha on Tue Mar 8 07:04:52 2011 said:
pito on Mon Mar 7 21:52:07 2011 said:
BioMiha on Mon Mar 7 20:36:02 2011 said:
pito on Mon Mar 7 12:51:27 2011 said:
pDNA on Sun Mar 6 11:27:02 2011 said:
I would prefere a company that is somehow near your lab (if possible on the same continent) ...so you do not have to send samples all around the world (allthough some people do send samples for sanger sequencing e.g. from Europe to Korea and that is a bit weird to me). Normally, sanger sequencing is a state of the art technique and read lengths >1000bp are more or less standard ...i think it is most important to purify the DNA in a good way because when it is of poor quality the sequencing will fail no matter what company you have choosen. Regards,p
BioMiha on Sat Mar 5 11:47:24 2011 said:
Macrogen
Dude, in my opinion you're just overdoing it a bit. I am all for eco, I just wanted to say there are better places to start, especially if saving a buck can help run a few additional sequences, get more results and eventually contribute in a small way. I would say going to work on foot or by bike instead of in your car or the subway saves more CO2 emissions than sending your samples to Germany instead of Korea. Not that I am in any way affiliated to the company, but they have cheaper prices because they have state-of-the-art robot sequencers not because they have cheap labor. We are talking about Korea, not China.
-pDNA-
BioMiha on Tue Mar 8 07:04:52 2011 said:
pito on Mon Mar 7 21:52:07 2011 said:
BioMiha on Mon Mar 7 20:36:02 2011 said:
pito on Mon Mar 7 12:51:27 2011 said:
pDNA on Sun Mar 6 11:27:02 2011 said:
I would prefere a company that is somehow near your lab (if possible on the same continent) ...so you do not have to send samples all around the world (allthough some people do send samples for sanger sequencing e.g. from Europe to Korea and that is a bit weird to me). Normally, sanger sequencing is a state of the art technique and read lengths >1000bp are more or less standard ...i think it is most important to purify the DNA in a good way because when it is of poor quality the sequencing will fail no matter what company you have choosen. Regards,p
BioMiha on Sat Mar 5 11:47:24 2011 said:
Macrogen
Dude, in my opinion you're just overdoing it a bit. I am all for eco, I just wanted to say there are better places to start, especially if saving a buck can help run a few additional sequences, get more results and eventually contribute in a small way. I would say going to work on foot or by bike instead of in your car or the subway saves more CO2 emissions than sending your samples to Germany instead of Korea. Not that I am in any way affiliated to the company, but they have cheaper prices because they have state-of-the-art robot sequencers not because they have cheap labor. We are talking about Korea, not China.
They have companies in europe (holland), japan and usa too... But do they sequence there too?
In the past: you could send samples to holland for free if you are from a european country.. but they didnt sequence it there, they just shipped it to korea too.....
You can say all you want: but its the same company, then why dont they have the same machine in europe (for ex) too? If its the machine itself....?
And what about other companies? WHy dont they have such machines? Is it because of patent laws or is it more then just the machine...?
And how can it be cheaper to send samples so far away? It doesnt add up really, I cant imagine that a flight to korea would be the same price as one to germany for ex (if you are in europe) or even if that the price to korea would be more expensive, that in the end the price of korea could still be soo cheap just because of the machine...
And I am not saying that Korea is like china or whatever , but its just weird, hard to understand how this is possible.
-pito-
Probably, over a longer period of time, it is cheaper to transport samples(across the globe) and process them automatically, than pay (local) staff for handling samples and process them automatically in the same region.
Also, installing imported machinery in a country might be an issue.
BTW, what is the turn around time for these companies, if the samples are shipped to Korea? The sequencing department (on the same floor)at my uni took 3 days.
-gt_ameya-
gt_ameya on Wed Mar 9 09:13:03 2011 said:
Probably, over a longer period of time, it is cheaper to transport samples(across the globe) and process them automatically, than pay (local) staff for handling samples and process them automatically in the same region.
Also, installing imported machinery in a country might be an issue.
BTW, what is the turn around time for these companies, if the samples are shipped to Korea? The sequencing department (on the same floor)at my uni took 3 days.
Good question about the turn around time. No idea. But ist faster then 3 days I think.
It does seem strange, being cheaper to send it so far.
-pito-