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DNA, Gel - Possible reasons for DNA Gel Smear (Feb/19/2011 )

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There is a lot of smear. It looks like a gel that I prepare when I want a smear.. lol

What protocol did you use? Did you use a beatshaker?


And did you check the sticky on this forum about how to prevent smearing

gt_ameya is also riht that you, even with the smear, still have some DNA bands.

-pito-

This is entirely typical of genomic DNA. Why do expect bands?

-phage434-

Thanks to all your reply.

yes, there was DNA and I did PCR with those DNA, there was some non-specific bands in the PCR Gel.


But I used another DNA extraction Kit (Powersoil Kit) and repeated DNA extraction again, it seemed better than before (little smear).

I am really confused that you can not see my pictures in attachment. What is the format of your picture uploaded? jpg? Thanks to all. :)

-sxh999-
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