Excitotoxity assay on primary hippocampal neurons - (Feb/18/2011 )

Primary hippocampal neurons seem to be quite insensitive to glutamate treatment. Does anybody have a protocol treating them in magnesium-free, glycine containing medium to increases their sensitivity (disinhibtion of NMDA receptors)?
Best regards,

The time of the experiment can influence the toxicity. Hippocampal neuron are quite insensitive to glutamte within the first 5 days in culure.
This experiment is usually done in Locke's solution and you have to add glycine separately. The quality of these solutions can make a difference.