Storing genomic DNA in 4C with PBS? - (Feb/15/2011 )

Hi all

I have been storing genomic DNA after a heat-lysis method in PBS. I have been doing this for some time and my PCR and sequencing seems to work fine. I am just worried that for my thesis this will cause me some mark deduction, as I did not use TE buffer, which is probably the conventional way of storing DNA. Has anyone stored gDNA in PBS?

Thanks

-PandaCreamPuff-

It should be fine, this is more or less the pH and salt levels inside the cell anyway... however, they are more prone to bacterial/fungal and DNAse digestion if stored in PBS.

-bob1-

bob1 on Tue Feb 15 21:10:26 2011 said:

It should be fine, this is more or less the pH and salt levels inside the cell anyway... however, they are more prone to bacterial/fungal and DNAse digestion if stored in PBS.

Thanks Bob.

-PandaCreamPuff-