Help with Isolation of protiens associated with lipid rafts - (Feb/03/2011 )
I am trying to determine if my protein of interest is localized to lipid rafts. I have been using (unsucessfully) a sucrose gradient protocol to do this but was wondering if there are better techniques out there. Does anyone have a lipid raft isolation protocol that consistantly works? Are sucrose gradients the best technique to use? Any help/advice would be greatly appreciated!
Thanks,
ABK
-DrABK-
you can find a protocol for the isolation from platlets here ...never did it on my own but i heard that it works fine!
Regards,
p
DrABK on Thu Feb 3 18:32:23 2011 said:
I am trying to determine if my protein of interest is localized to lipid rafts. I have been using (unsucessfully) a sucrose gradient protocol to do this but was wondering if there are better techniques out there. Does anyone have a lipid raft isolation protocol that consistantly works? Are sucrose gradients the best technique to use? Any help/advice would be greatly appreciated!
Thanks,
ABK
-pDNA-