PBMC Culturing - (Jan/31/2011 )
I have received several sets of PBMC that are infected with HIV from NIH. Their propogation protocol is skimpy at best. They suggest growing the cells in RPMI-1640 media supplemented with 10U/mL of IL-2. I have tried this with 2 sets of cells and each time the culture died. Could someone suggest a protocol that would allow me to propogate PBMCs??? Thanks!
-Deepe-
what kind of cell you need from PBMC.
based on your requirement you can use different type of supplements
-beny-
Did the product info sheets that came with the cells recommend a manufacturer for the IL-2? If so, it would be worthwhile to try obtaining the IL-2 from the same place.
Just a thought...
Labrat612
-labrat612-