HPLC column cleaning - (Jan/30/2011 )

Hi everyone,

I am about to start a new HPLC assay using a different mobile phase from what I have used before. The new mobile phase will be
acetonitrile:water, 50:50 v/v, with 25mM citric acid and 10mM sodium dodecyl sulfate added. I have never added citric acid or SDS to my mobile phase, so I am not sure if there are any precautions I need to take in order to protect my column. The pH of the mobile phase is expected to be approximately 2.0.

The column I will be using is an Apollo C18, 5 um (250mm x 4.6 ID), with an optiguard C18 pre-column.

I am not sure if I need to clean/rinse my column with anything after using it.

Any thoughts would be appreciated.

Thanks!

hi
adding SDS to a RP column can be tricky, it can end up spoiling the column so be cautious.

Hi,

Thanks for your response. When you say SDS can spoil the column, does that mean that I may not be able to use that column for other assays, but it will still be ok for the assay using SDS? I have read some information that alludes to this, but nothing concrete.

Thanks again.