Protocol Online logo
Top : New Forum Archives (2009-): : PCR, RT-PCR and Real-Time PCR

Question of nucleic acid extraction - (Jan/28/2011 )

When performing RNA extraction using spin-column method, could RNA pellet be reconstituted or not? I'm not sure.

-cike2008-

I theory yes, you can resuspend a RNA pellet, but my recommendation is not to do it. By the time you get it resuspended it will most likely be degraded.

-ivanbio-