Osmolarity - (Jan/18/2011 )
This solution
160 NaCl
2.5 KCl
1 CaCl2
2 MgCl2
10 HEPES
10 Glucose
has an osmolarity of about 320, how to calculate it from the molarity of the ionc composition.
-samita-
I think itīs more in the range of 350 mOsmol...
samita on Tue Jan 18 17:08:32 2011 said:
This solution
160 NaCl
2.5 KCl
1 CaCl2
2 MgCl2
10 HEPES
10 Glucose
has an osmolarity of about 320, how to calculate it from the molarity of the ionc composition.
-Inmost sun-
Think about it like this... 1 mole of NaCl goes into solution to form one mole of Na+ and one mole of Cl- = 2 Osmole... HEPES and glucose do not do this so the osmolarity is 1 for each mole dissolved.
Assuming your solution above is in millimoles/litre (you should always write solutions with concentration units)
160 mM NaCl = 320 mOsmole
2.5 mM KCl = 5 mOsmole... etc I am sure you get the picture.
BTW Inmost sun is correct...
-bob1-