difference between PCR primers and sequencing primers - (Jan/16/2011 )
Hi
Is there any differences between primer design rules for PCr and sequencing reactions?
actually I design some primers for sequencing according to my experience on pcr primer design but it didnt work, so is there any special issue in designing sequencing primers?
regards
-sara.r-
You can often use the primers you used for PCR for sequencing reactions, however some will work much better than others (don't really know why). I would try and when it doesn't work, I'd redesign internal primers.
What is the annealing temperature of your PCR?
-Maddie-