establishment of primary cell line from biopsy - (Jan/04/2011 )

Hi everyone,

I'm trying to establish primary cell lines from bladder cancer samples. I keep trying, but after a while I still cant have any growth at all!

I've been using this protocol where I cut the sample into little pieces, seed it and then add some F-12 medium + non essencial a.a., dextrose, insulin, glutamin, P+S as antibiotics and FBS.

Cant really understand where is the problem, no article describes this problem or how to fix it, im really getting worried.

Thank you in advance
Happy 2011 everyone

Nya

Nya on Tue Jan 4 15:11:55 2011 said:

Hi everyone,

I'm trying to establish primary cell lines from bladder cancer samples. I keep trying, but after a while I still cant have any growth at all!

I've been using this protocol where I cut the sample into little pieces, seed it and then add some F-12 medium + non essencial a.a., dextrose, insulin, glutamin, P+S as antibiotics and FBS.

Cant really understand where is the problem, no article describes this problem or how to fix it, im really getting worried.

Thank you in advance
Happy 2011 everyone

Nya

Thanks so much for your suggestion!