primary astrocyte culture - HEEEELP! (Dec/21/2010 )

hello,

I am a young scientist and for about a year now I have been trying to optimize my protocol to establish primary newborm rat astrocyte cell cultures.
The procedure an sich is not a problem, I am just having lots of problems due to infections.

I used have a lot of fungal infections but this problem seemed to be solved by adding some amphotericin B in my culture medium.
Today I discovered a yeast infection. This is the first time I encouter this. Does anyone know what it is coming from, how to avoid it or even how te clean my cultures from it?

many thanks

ps: is it true that it might be due to the fact that I just started to bake my own bread? I keep on reading this on some fora

possible. or even of you drink beer and work with cells, yeast infection is possible.

try to work aseptic as possible.