ELISA with murine serum - (Dec/16/2010 )

Hi!
I will start a new protocol for detection of IL-6 following stimulation with pam3Cys-sk4 in mouse serum.
Does anyone has already did it?
I don't know what dilution to do with my serum?
Many thanks if someone can help me!

http://www.biolegend.com/index.php?page=pro_sub_cat&action=search&criteria=il-6&gclid=CN3viMi186UCFUmo4Aodnmugog

http://www.biocompare.com/ProductListings/28718/Mouse-IL-6-ELISA-EIA-Kits.html

Kits already on market see above links.