cell culture clarification - I need help please (Dec/13/2010 )

I am a chemist who has gotten roped in to doing cell culture at work. I have to purify media collected from cells and I have never done it before. I have a protocol from a journal to work with. The cells have grown and media has been collected which I now need to purify.

journal says "medium...was collected and clarified at 3000rpm in a Sorvall RT6000 refrigerated centrifuge for 10min at 4C." I do not know what is meant by "clarified" Do I use a special centrifuge tube with a filter? or am I over-analyzing this and it simply means to spin down? Or is there something I put in the centrifuge tube?

Thanks for any help

-Dawn M-

Dawn M on Mon Dec 13 19:28:01 2010 said:

or am I over-analyzing this and it simply means to spin down?

That.

In this context "clarification" means "removal of bigger debris" - eg. cells, cell fragments etc.

-K.B.-

haha thanks! I thought so. it just seemed too easy

I was also wondering what they meant by 'adjusting the supernatant to...' but then realized they just meant added

-Dawn M-