Minimum number of cells needed for cytometry? - (Dec/11/2010 )
In order to determine transfection efficiency for GFP plasmids, I'll like to count cells using a flow cytometer but my supervisor says it will take too long to expand our cells to a sufficient quantity. Why would there be a minimum number of cells needed to run through a cytometer?
-seanspotatobusiness-
Usually FACS uses 10,000 events as a positive cut off for statistical purposes - you should be able to get this many cells out of a t-25 flask easily.
-bob1-
bob1 on Sun Dec 12 22:01:41 2010 said:
Usually FACS uses 10,000 events as a positive cut off for statistical purposes - you should be able to get this many cells out of a t-25 flask easily.
Is that a T-25 flask of adherent cells? Mine are suspended and currently occupy wells in 96-well plates (300 ul medium).
-seanspotatobusiness-
One well of a 24 well plate gives you enough cells for FACS. I don't know about suspension cell culture though... What is your cell density?
rsm
-Rsm-