Differentiation of CD11b cells into macrophages.Help please - (Dec/03/2010 )

Hi,

I would like to differentiate CD11b monocytes (isolated from mouse) into macrophages. Some papers described that treatment for 24h with 20nM of PMA of THP-1 cells will induce a macrophage phenotype. Does it work also for CD11b monocytes or is there a better/standard treatment?

Thanks a lot

GM-CSF and M-CSFare the two growth factors giving rise to monocyte/macrophage-restricted progeny.

http://nic.sav.sk/logos/books/scientific/node21.html