single gene amplification - cDNA synthesis and amplification with gene specific primers (Dec/03/2010 )

I'm trying to amplify the protein kinase gene involved in drought tolerence; i've checked my rna with gel, and I also amplified cDNA. The PCR product is showing high amount of primer dimers and the band is not very much distinct. still i have eluted and reamplified that band but still I AM GETTING PRIMER DIMER AND VERY LESS CONCENTRATION. so can be the reason whether my primer desigfning ok any other. please anybody reply....
Thanks anyway in advance

you may want to redesign your primers and check them for dimer formation before ordering them.

mdfenko on Fri Dec 3 15:53:50 2010 said:

I got a good band and less primer dimer after diluting primer. I think my primer works well. Anyway thank you for sugestion.