IC50 - (Dec/01/2010 )

Hey guys

Could one of you geniuses help me with this. Ive been given a table with inhibitor drug concentrations and another two column with enzyme activity in the presence of either drug. I need to calculate IC-50 values. Ive figured I need to draw a graph. What do I plot on the graphs?! and also..what can I comment or infer after seeing the values?

Thanks!
- Mon -

Hello monica_bio,

read this : evaluation of enzyme inhibitors in drug discovery

pay attention for IC50 the graph is sigmoidal.
i.e : the X-axis is log ; X : conc.

and here is a software : GraphPad Prism Free Download
and here, you can read about it : GraphPad Prism Scientific graphing
explore the site, there is a free demo ...

hope beneficial !

All The Best.

Until now, I have a vague understanding in determining ic50. Different software gave different values. What is the minimum point for accurate IC50 determination. In my cases, the inhibitory effect at low concentration will vary between replicates and not at high concentration. When all points were selected, the varied value at lower concentration will make large difference of linear equation between replicates. When I chose only three points, I got good result with small SD. I dont know this is acceptable as I cant find any articles specifically discuss about ic50 determination

kentz19 on Fri Dec 3 12:26:23 2010 said: