Sequencing mistake - (Nov/12/2010 )

I just send my gene from mutation PCR for sequencing and have one mising base pair in the sequence....
I have attacged the picture and it seems to me G is there but peak is not visible...
or its deletion mutation from polymerase by itself?????

I need to see a few more surrounding peaks to be able to tell anything. And it would be good to know what the before and after mutation sequence was supposed to be.

Thanks for your reply...
I have attached the new picture
alignment
mutant= 5-GAC-TAC-3
WT = 5-GACCTAC-3
I used the reverse complementary sequence from the sequncing file to align with wild type...

I can't interpret the upper trace in your picture. The bottom trace appears to correlate with the called sequence, but the base caller made an error, I believe. The sequence around the yellow region should read ccgtaggtcct (there is an omitted g, because the g peak does not appear to be quite wide enough, although from the spacing it is quite clear that there is a second g.

I assume this is the problem you are worrying about -- although I still can't make any sense out of the top trace.
If I read things correctly, the answer is "don't worry, be happy."