Is it possible to know only the live bacteria using PCR for a certain genus? - (Nov/06/2010 )

I am doing starvation experiment for nitrifying bacteria in wastewater and I want to know only the live bacteria for nitrobacter and nitrosospira for example. I am using T-RFLP. The normal PCR will amplify both the live and the dead bacteria DNA as I thank. Any suggestions

Abdelsalam Elawwad on Sat Nov 6 21:08:42 2010 said:

FACS sorting. Don't know if you have, but would work.

rsm