Quickchange mutagenesis primer - (Nov/04/2010 )

Hi,
How do I design quickchange mutagenesis primer?

thanks

Agilent has a tool to aid in the design of QuickChange primers -- see here.

i used this and worked really well...try this.

http://www.bioinformatics.org/primerx/documentation.html

Gnana..

I did manually for a fragment deletion