How to use the loading control? - (Oct/30/2010 )
I've run a WB with beta-actin as the loading control. I've quantified the actual protein I was looking for but I don't actually know what I'm supposed to do with the loading control. Am I meant to quantify that as well? Why? Help would be appreciated!
-ashamon-
How did you quantify? You must have quantitated relative to something, if it wasn't relative to the loading control, then you seem to have mis-understood the quantitation process.
-bob1-
Ah ok - after obtaining a value for each protein band, was I supposed to make it a proportion to the value on their respective loading control band?
-ashamon-
Yes, exactly right.
-bob1-