Longer incubation in primary antibody - (Oct/30/2010 )

Hi!
I incubated my blots in primary antibody for more than 24 hrs in 2.5% Milk at 4 degree.
One of my primary is notorious for non specific binding while the others are pretty neat.
Will longer incubation in primary give me a lot of nonspecific binding and background?
Any suggestion will be highly appreciated.

No worries, it should be fine. Just wash well with TBST 3x10 min.

Curtis on Sat Oct 30 17:24:02 2010 said: