clone GENE RT-PCT PRIMERS - cloning genes (Oct/28/2010 )
hi....
If i have the mRNA sequence of a purfied protein, and want to clone a specific cDNA for thaumatin protein (So need thaumatin from the mRNA i already have but don`t know what encode thamatin) via RT-PCR. How can I design a specific primer for thaumatin from all the mRNA sequence.
THANKS
-BILLO-
what do you have?
Do you know the DNA sequence of your protein?
Do you know the AA sequence of your protein?
-perneseblue-