midiprep - plasmid extraction (Oct/25/2010 )

Hi,

I've eluted my plasmid using midi-prep and the concentration is 1800ng/ul. I need to trafect my cells with 6ug using lipofectamine. Just out of curiosity, is there a problem using too concentarted plasmid?

SF_HK on Mon Oct 25 07:14:09 2010 said:

According to invitrogen manual (http://tools.invitrogen.com/content/sfs/manuals/lipofectamine2000_man.pdf) you will add LFM when you diluted DNA to a certain concentration. It works similar for other manufacturers. If you follow the protocol you should be good;)