6.7%Polyacrylamide/50% Urea gel - (Oct/18/2010 )

Hi,

very new to this thing and need help,

is there anyone know how to do 6.7%Polyacrylamide/50% Urea gel

Many thanks.

what buffer are you using? what crosslinker concentration?

to 50gm urea, add buffer and acrylamide (with crosslinker) concentrates and water to 100ml.

stir (without heating, urea will decompose) untill all the urea is dissolved (it will take some time because the temperature will drop).