DNA isolation from human serum/plasma - (Oct/12/2010 )
Hi!
Has anyone ever worked with human serum or plasma to extract the DNA?
I am interested in the kit you've used and the amount of plasma/serum you need to get a reasonable amount of DNA...
I want to do some SNPs identification with enzyme digestion afterwards...
any information would be very useful....
thanks!!!
-kitty03_1978-
kitty03_1978 on Tue Oct 12 21:00:34 2010 said:
Hi!
Has anyone ever worked with human serum or plasma to extract the DNA?
I am interested in the kit you've used and the amount of plasma/serum you need to get a reasonable amount of DNA...
I want to do some SNPs identification with enzyme digestion afterwards...
any information would be very useful....
thanks!!!
I would just do an ethanol precipitation just as you would do with PCR reactions. Maybe a PCR-prufication Kit works also. You should just try.
-Montys-