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DNA isolation from human serum/plasma - (Oct/12/2010 )

Hi!
Has anyone ever worked with human serum or plasma to extract the DNA?
I am interested in the kit you've used and the amount of plasma/serum you need to get a reasonable amount of DNA...

I want to do some SNPs identification with enzyme digestion afterwards...
any information would be very useful....
thanks!!! ;)

-kitty03_1978-

kitty03_1978 on Tue Oct 12 21:00:34 2010 said:


Hi!
Has anyone ever worked with human serum or plasma to extract the DNA?
I am interested in the kit you've used and the amount of plasma/serum you need to get a reasonable amount of DNA...

I want to do some SNPs identification with enzyme digestion afterwards...
any information would be very useful....
thanks!!! ;)


I would just do an ethanol precipitation just as you would do with PCR reactions. Maybe a PCR-prufication Kit works also. You should just try.

-Montys-