Viral DNA extraction - (Oct/12/2010 )
Hi bioforumers
Iam startig to wrk in a new project,my first task is to do PCR from lyophilized varicella vaccine (Oka strain)
Should I do direct PCR or DNA extraction and which protocol should I use
-desertrose-
DNA extraction should give better (as in more consistent) results. A standard phenol-chloroform preparation should work well.
-bob1-