cell cycle arrest by colcemid - (Oct/08/2010 )
I stain my cells with CFSE to analyze their cell cycle activity and as a positive control, I would like to culture the cells under the presence of colcemid which inhibts the cell cycle
I cannot find any information on the stability of colcemid in culture (37°, pH 7.4) . How often do I have to refresh the colcemid-medium, so that cell cycle is efficiently inhibited?
-ija0-
I've never used Colcemid, but I use Mitomycin C in medium. Could you use it as an equivalent?
ija0 on Fri Oct 8 09:52:01 2010 said:
I stain my cells with CFSE to analyze their cell cycle activity and as a positive control, I would like to culture the cells under the presence of colcemid which inhibts the cell cycle
I cannot find any information on the stability of colcemid in culture (37°, pH 7.4) . How often do I have to refresh the colcemid-medium, so that cell cycle is efficiently inhibited?
-aleruiz-
ija0 on Fri Oct 8 09:52:01 2010 said:
I stain my cells with CFSE to analyze their cell cycle activity
???? Cell cycle by CFSE?
May be "to analyse cell divisions"?
-Denis Baev-