colony, focus or embryoid body? - (Oct/03/2010 )
Dear all, I am culturing the embryonic stem cells of zebrafish. My cells start to attach and form big colonies although I swirl the plate after seeding. Now, I worry that the colonies might be cancer cells, but I don't know how to distinguish them between normal colonies and cancer cells. Besides, what is the differences between embryoid body and normal forming colonies? Can embryoid body formed spontaneously?
p/s: I am using liquid medium with LIF free, the well is coated with 0.1% gelatin, and feeder free.
Thank you!
I need somebody answer me....please help me
needs some infor about howu subculting these cells
from r note, it is clear that the ES cells since grown at high density spotaneously forms 3d structures -EBs which a way to say that they undergoing differentiation.
enock02 on Tue Oct 5 13:50:24 2010 said:
I need somebody answer me....please help me
enock02 on Sun Oct 3 08:12:57 2010 said:
Dear all, I am culturing the embryonic stem cells of zebrafish. My cells start to attach and form big colonies although I swirl the plate after seeding. Now, I worry that the colonies might be cancer cells, but I don't know how to distinguish them between normal colonies and cancer cells. Besides, what is the differences between embryoid body and normal forming colonies? Can embryoid body formed spontaneously?
p/s: I am using liquid medium with LIF free, the well is coated with 0.1% gelatin, and feeder free.
Thank you!
Hi enock
I don't know much about zebra fish stem cell markers, but you could just stain them to be sure that you have the right cells (a picture would also be nice).
In the meantime check these papers out:
http://www.ncbi.nlm.nih.gov/pubmed/17141029
http://bfg.oxfordjournals.org/content/1/2/131.full.pdf
If you can't get them PM me.
Best regards
Radish