lentivirus for invitro study - (Sep/15/2010 )
Hi
Does anyone know if i can use the media supernatant of my lentivirus for infecting cells (in vitro experiments) or i have to follow the complete protocol of purifying and concentrating the virus and then using on cells...i know for in vivo studies i need the virus to be pure and conc. but is it required for the in vitro stuff too????
-satty-
satty on Wed Sep 15 20:05:01 2010 said:
Hi
Does anyone know if i can use the media supernatant of my lentivirus for infecting cells (in vitro experiments) or i have to follow the complete protocol of purifying and concentrating the virus and then using on cells...i know for in vivo studies i need the virus to be pure and conc. but is it required for the in vitro stuff too????
Yes, you can use it directly
-laurequillo-