what is the optimal temperature for my restriction enzyme - Urgent plea (Sep/14/2010 )

What is the optimal temperature to use for my RE Xba1 ??

Thanks, I haven't use this before yet..

Hola, Check any catalogue and it shows all the specifications of each RE. I have seen a Takara poster and it says 37ºC with media salt buffer and BSA. Good luck

I can give u the optimum temp for XbaI but if u want to check for other enzymes in future, thatswhat i am suggesting u to check in the neb site.

Temp is 37 (http://www.neb.com/nebecomm/products/productR0145.asp) Check the link.


And one more thing about XbaI is the flanking bases of the restriction site (dam methylation). I had a bitter experience, after that i changed the flanking and worked out well.

All the best.

ocean_sky83 on Tue Sep 14 05:44:58 2010 said:

When you buy the enzyme it comes with little info sheet how to do the digestion