lysis of e.coli by sonication - (Sep/11/2010 )

For 10ml culture volume what are the sonication conditions to be maintained, cn anybody plzz help mw out

bhavana on Sat Sep 11 16:52:24 2010 said:

20 cycles of 30 min with amplitude of 60 will be enough to lyse cells completely, give a break of 1 minut after each cycle

Is it 20 cycles of 30sec or 30 min ?