gel filtration.. - (Aug/23/2010 )

hi...
can somebody help me to calculate how much bio gel p-100 i need to run gel filtration..
it is necessary to calculate the gel need from the bradford result??

in classical lc, gel filtration is generally performed in columns that are 50 or 100 cm long (depending on the desired or necessary resolution). knowing how much protein you have can help you decide what diameter column you require for adequate resolution.

you need to use enough gel to fill the column to an adequate height after proper packing.