allium sativum (garlic) and its antioxidant - (Aug/10/2010 )

can sumbody help me, how to find antioxidant gene in garlic?
i try to search at NCBI, but there is a lot of gene about garlic come out..
i dono which one code for the antioxidant which is allicin.. i hv to order primer from it..

As far as I can see there is no gene for Allicin, it is produced by the enzyme Alliinase acting on Alliin which is a modified cysteine. There are two entries on NCBI for Allicin production from bacteria and fungus respectively.

bob1 on Tue Aug 10 23:01:22 2010 said:

the production is in wut category? nucleotide or jurnal? how am i going to get the sequence of allinase production?

http://www.ncbi.nlm.nih.gov/gquery/?term=ALLICIN Have a look at the Nucleotide link.

bob1 on Wed Aug 11 22:25:10 2010 said:

yeaaa!! got the sequence! it codes for allinase rite?
but why the source of organism is fusarium?
when i blast the sequence, it comes out the sinorhizobium, wuts their correlation?
i hv another problem, when i click for pick primer, it says that "Primer specificity cannot be properly determined as sequences from the specified organism (Fusarium sp.) are not present in selected database: refseq_rna. "

should i go to protein sequence instead of nucleotide.. cuz i hv to do pcr for gene responsible codes for allinase? or should i search for allin? its kinda confuse

Those genes are ones from Fusarium, which is a fungus (sinorhizobium is a bacterium) and will not be the same as the one found in garlic, though there may well be some similarities.

Try http://www.ncbi.nlm.nih.gov/nuccore?term=alliinase+AND+%22Allium+sativum%22 for the alliinase genes. Look for complete cds.

-bob1-

bob1 on Thu Aug 12 20:48:04 2010 said:

ok thx.. i saved dat sequnce.. but now i want to find sequence for alliin.. when i search allin at NCBI, it comes out the allin lyase.. from what i know, allin lyase is another name for alliinase rite?

-ikwana-