Calculating osmolarity - (Aug/05/2010 )

anyone who know a program to calculate buffer osmolarity from molarity of ingredients?

might help

http://www.ehow.com/how_6316424_use-molarity-calculate-osmolarity.html

thanks prep! your links shows indirectly what the problem is: The author suggests that all ingredients fully dissociate but some buffer ingredients, f.i.some phosphate salts, do not; so,the dissociation constants must be regarded to get the real osmolarity...

Inmost sun on Sat Aug 7 22:46:20 2010 said:

wikipedia gives the formula. Question is if you have the osmotic coefficient for all chemicals of the buffer. And it depends on the temperature...