PMSF solution - (Aug/03/2010 )
I need to make 100 mM PMSF solution. I dissolve require amonut in 20 ml and shae it so that PMSF get dissolve but when i store it, the solution become turbid. I can see the crystal of PMSF suspended in isoproponal.
how to make solution and store it form PMSF????/
Where are you storing your PMSF? I have always diluted in EtOH and stored at 4degC (protected from light) and it's fine.
Clare
samita on Tue Aug 3 10:05:28 2010 said:
I need to make 100 mM PMSF solution. I dissolve require amonut in 20 ml and shae it so that PMSF get dissolve but when i store it, the solution become turbid. I can see the crystal of PMSF suspended in isoproponal.
how to make solution and store it form PMSF????/
I am preparing in isoproponal and previously I was putting at 4 but now I am storing at -20, I was advise to do this.
Clare on Tue Aug 3 12:35:04 2010 said:
Where are you storing your PMSF? I have always diluted in EtOH and stored at 4degC (protected from light) and it's fine.
Clare
samita on Tue Aug 3 10:05:28 2010 said:
I need to make 100 mM PMSF solution. I dissolve require amonut in 20 ml and shae it so that PMSF get dissolve but when i store it, the solution become turbid. I can see the crystal of PMSF suspended in isoproponal.
how to make solution and store it form PMSF????/
Sigma suggests storage at 4degC.
http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/1/p7626pis.Par.0001.File.tmp/p7626pis.pdf
Why don't you give that a go?
Clare
samita on Wed Aug 4 09:36:21 2010 said:
I am preparing in isoproponal and previously I was putting at 4 but now I am storing at -20, I was advise to do this.
Clare on Tue Aug 3 12:35:04 2010 said:
Where are you storing your PMSF? I have always diluted in EtOH and stored at 4degC (protected from light) and it's fine.
Clare
samita on Tue Aug 3 10:05:28 2010 said:
I need to make 100 mM PMSF solution. I dissolve require amonut in 20 ml and shae it so that PMSF get dissolve but when i store it, the solution become turbid. I can see the crystal of PMSF suspended in isoproponal.
how to make solution and store it form PMSF????/