PCR AMPLIFICATION - (Aug/03/2010 )
HI FRIENDS
I AM A PLANT MOLECULAR BIOLOGY STUDENT. I HAVE PREPARED THE GENE SPECIFIC PRIMERS. MY PROBELEM IS THAT I GOT AMPLIFICATION WITH cDNA BUT UNABLE TO GOT FROM THE GENOMIC DNA. i AM UNABLE TO TROUBLESHOOT THIS.
-PRATIPAL-
PRATIPAL on Tue Aug 3 05:15:18 2010 said:
HI FRIENDS
I AM A PLANT MOLECULAR BIOLOGY STUDENT. I HAVE PREPARED THE GENE SPECIFIC PRIMERS. MY PROBELEM IS THAT I GOT AMPLIFICATION WITH cDNA BUT UNABLE TO GOT FROM THE GENOMIC DNA. i AM UNABLE TO TROUBLESHOOT THIS.
Did your primer spans the intron region, and only amplified the spliced exon?
-adrian kohsf-