buffer storage - (Aug/02/2010 )

How long would you store the following buffer (4 C):

60 mM Tris
2 mM EDTA
2 mM EGTA

pH 8,1 ?

I still have quite a lot in the cold room and I don't know if it is of any use after one year.

Thanks for help!

j

Hard to believe anything is growing in it. It's probably ok.

phage434 on Mon Aug 2 12:59:17 2010 said:

I don't think anything is growing there either, at least macroscopically it looks clean.

What about other issues of stability etc?

all three components are stable. you can check the pH to ensure that there is no significant variation but make sure you check at the same temperature that you originally adjusted.