why my protein precipitates? - (Jul/28/2010 )

1) i am trying to crystallize my protein and for that i lyophilized my protein sample. After obtaining in powder form, when i tried to redissolve it in water, it precipitated. Why?

2) I have another batch's protein where i think lyophilization was a bit incomplete in the sense that it still is flaky, this one behaves very transparent upon redissolving. Why?

Can somebody throw a light?

Thanks
Parveen

Hi,

Maybe during the lyophilization process your protein changed its structure, you can try redisolved in DMSO and water.

parveen on Wed Jul 28 18:46:40 2010 said:

think "degree of hydration". just like with nucleic acids, some proteins don't like to be dried completely.