Lactic acid bateria maintenance - (Jul/21/2010 )

HI all,

We have procured few lactic acid bateria for trials from ATCC.

we would like to preserve them for long period by storing them at -80º or in LN2. we normally prepare glycerol stock of cultures to store them for long periods. But or lactobacilli skim milk is recommended in some papers and i am confused. should i grow the culture in skimmed milk nd preserve it in that or should i grow them in MRS and then preserve in skim milk or go for glycerol stock preparation.


can anyone guide me with a protocol or methodology for long term preservation of lactic acid bacteria.

thanks in anticipation

There are many papers ...


just search...

and didnt the ATCC give you any information?

Just to help you look:

http://www3.interscience.wiley.com/journal...=1&SRETRY=0

http://www.springerlink.com/content/lp8gl25317260875/

http://www3.interscience.wiley.com/journal...850816/abstract