Cheap color filters? - Cellophane? Wavelengths? (Jul/14/2010 )
Or if someone has in your department a UV/VIS spectrometer or somewhere else you can rent it. It should be something like this one here
, though there are many companies and lots of different machines, mostly unfortunately not cheap...
-hobglobin-
K.B. on Jul 15 2010, 04:11 PM said:
Which model of Nanodrop do you have? 1000 or 2000? First one has path length between 0.2 and 1.0 mm, the other - 0.05-1.0 mm. I'm pretty sure you can make this measurement with Nanodrop 1000 but I would not risk it with Nanodrop 2000 (unless you know the thickness of cellophane and it's less than 0.05 mm).
If you want to do it - pick "UV-Vis" from the main menu, zero on water, place piece of cellophane (eg. 5x5mm or smaller) on the top of drop of water on Nanodrop pedestal, place another drop of water on the top of cellophane, lower the upper pedestal (lid/arm) and measure.
If you want to do it - pick "UV-Vis" from the main menu, zero on water, place piece of cellophane (eg. 5x5mm or smaller) on the top of drop of water on Nanodrop pedestal, place another drop of water on the top of cellophane, lower the upper pedestal (lid/arm) and measure.
KB, that's exactly what I needed to know. Thank you! I'll give this a shot once I get my hands on the cellophane now that I know how to test it (and as long as the lab that shares it with us is ok with it).
-DustinL-