Primer design for qPCR - (Jul/09/2010 )

Hi all,
could anyone tell me how to design primers for qPCR?
I'm not very good in bioinformatics...
Thanks!

Hello,

I did a lot of qPCR work lately and I had very good results with the rtprimerdb (http://medgen.ugent.be/rtprimerdb/) and primerquest (http://eu.idtdna.com/Scitools/Applications/Primerquest/Default.aspx) tools.

With rtprimerdb, you can, however, design only 5 primer pairs per 24 hours.

If you have any other problems, feel free to let me know!

Regards,
Philip

borriello.87 on Jul 9 2010, 02:52 PM said:

Hi all,
could anyone tell me how to design primers for qPCR?
I'm not very good in bioinformatics...
Thanks!

I can suggest you this one "getprime": http://updepla1srv1.epfl.ch/getprime/ It combines all features you need to have good primer quality and specificity (do not need to blast!). I get very good primers with this design.

Good luck!