Loading on PAGE - (Jul/08/2010 )
Any body has any idea on why DNA samples loaded on Native PAGE dont migrate all at the sam level?why this would happen? any suggestion?
-Fhannan-
different size, salt content of the sample, tilted gel (not level),...
show us a picture
-mdfenko-
No, they are all with the same size see the attached... Is it because the running buffer is not ok or APS is not fresh enough?
-Fhannan-
bubbles under the center of the gel (between the plates) could cause this.
-mdfenko-