Spores separation from fermentation broth - Spores (Jul/07/2010 )

I am working on the spores' separation from fermentation broth. The broth contains suspended and dissolved nutrients. Many spores stick to these solids and centrifugation by sucrose solution can NOT separate them.

Does anyone have idea how to purify these spores? Or how to remove these adherent spores from dissolved nutrients?

Any suggestions would be greatly appreciated.

supposing you are working with fungal spores I would try (i) sonication or (ii) adding some mild detergent. For most fungi this should work (if the spores are not already germinated and grown onto the nutrients..

try to use if glusalase treatment can be used on your species of fungus? In fission and budding yeast, this enzyme is used to digest vegetative cells but it leaves spores intact.

Thanks for your suggestion. I tried sonication before and it didn't work. Maybe the spores are already grown onto the nutrients (mainly suspended protein). Could you please give me an example for the mild detergent you mentioned?
Thanks again.

gebirgsziege on Jul 7 2010, 11:42 PM said:

Thanks. I am going to try.

perneseblue on Jul 8 2010, 04:45 AM said:

digestion on Jul 8 2010, 06:31 PM said:

Thanks a lot.
If the spores are already grown on the suspended nutrients, any suggestions to separate? Thanks again.

Tween 80 is our detergent of choice (final concentration 0.01%)