qPCR efficiency calculation - (Jul/04/2010 )
Hello everyone.
I would like to know how to calculate the efficiency of the qPCR. The slope value is -3.46. Please help
-chn09-
hi,
the formula of the efficiency (in %) is E(%) = (10-1/slope -1) *100. so for your slope this should be 94.5 % which is pretty good
-tea-test-