how can i bring cells onto a slide? - ...for microscopical observation! (Jul/01/2010 )

how can i bring cells onto a slide without cytocentrifugation?

thanks for recommendation

moljul on Jul 1 2010, 01:22 PM said:

you can seed them on the top of a cover glass ( I am not sure if that is your question...).

laurequillo on Jul 1 2010, 04:40 AM said:

not really feasible. i want to observe my macs sorted cells under the microscope, therefore i have to bring them onto a slide!

If you just want a quick look at your cells you can just drop 10 µl onto a glass slide and put a coverslip over the drop. Then you can view them under the microscope

SuMi on Jul 1 2010, 10:09 AM said:

in that case they have to be as concentrated as possible??

No not at all. I did it last week with 10 µl used directly after separation which included a wash through with 500 µl buffer so they were quite dilute

moljul on Jul 1 2010, 11:22 AM said:

as suggested, seeding on cover glas is a good idea; round coverglas is to prefer for round wells of cell culture plates; if cells do not grow on glas, try coating with collagen, fibronectin, matrigel etc

Inmost sun on Jul 2 2010, 09:13 PM said:

Or poly-l-lysine. I did not get why you can not seed them. But anyway, of course you can just take a look directly from the flask, just puting some of them in a cover. But if you dont need to stain them or anything you can just check from the plate or the flask in the microscope. You just put the plate there and check whatever you are interested in (gfp or morphology or whatever...)