Annexin and propidium staining - (Jun/25/2010 )

Does any one work with these apoptosis markers in lymphoblasts, i just need to knw the procedure

thanks in advance!

Follow the manufacturer's recommendation for Annexin V (use the right buffer etc), and add PI to a final conc of 50ng/ml. Use unstained - only AnnV - only PI as negative control and for compensation.

Cheers,

Minna